(A) Sequence alignment of human CALHM3, CALHM2, and CALHM1, and of murine and C. elegans CALHM1. Conserved sequences are highlighted in blue and sequence conservation is mapped in a color gradient, the darkest color representing sequences with absolute identity and lighter colors representing sequences with weaker conservation. Boxes denote hydrophobic domains 1–4 (HD1–4). Stars, predicted N-glycosylation sites on human CALHM1.
(B) Phylogenetic tree including human CALHM1 (hCALHM1).