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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Exp Hematol. 2008 Apr 8;36(7):832–844. doi: 10.1016/j.exphem.2008.02.009

Figure 1. Expression of CaM kinase transcripts in multiple models of neutrophil differentiation.

Figure 1

(A) Northern blots were generated using total RNA (5 µg) from uninduced EML and EPRO cells, and EPRO cells induced with 10 µM ATRA for 48 hours. Blots were sequentially hybridized with 32P-labelled cDNAs for the kinase shown and mouse β-actin as a control. (B) Northern analyses were performed using total RNAs from uninduced EPRO cells and cells induced with ATRA for 2 and 4 hours (left panels), or for 24, 48 and 72 hours (right panels). (C) A northern blot was generated using total RNA from SCF ER-Hoxb8 cells grown in the presence of β-estrodiol (1 µM, time 0), and cells grown after β-estrodiol withdrawal to induce neutrophil differentiation (48–96 hours). The blot was sequentially hybridized with 32P-labelled cDNA probes for the two kinases, lactoferrin (to demonstrate neutrophil differentiation) and β-actin. (D) Expression of CaMKKα and CKLiK were assessed in bone marrow-derived stem cells induced with SCF, IL-3 and G-CSF for 3 and 5 days, and then cultured in G-CSF alone. (E) A northern analysis identifies the expression of human CaMKKα and CKLiK in ATRA-induced NB-4 cells.