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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Exp Hematol. 2008 Apr 8;36(7):832–844. doi: 10.1016/j.exphem.2008.02.009

Figure 4. Expression of constitutively active CaM kinases inhibits the respiratory burst in differentiated EPRO cells.

Figure 4

(A) EPRO cells expressing CKLiK385-CA demonstrate reduced levels of the respiratory burst as compared to cells expressing the empty vector. Assays were performed on ATRA-induced cells stimulated with PMA (4 µg/mL) using an enhanced luminol reagent to detect O2− production. Shown are the percentages of peak light units emitted over 10 sec. during a 5 min. period of analysis as compared to control cells expressing the empty vector, set at a 100% response. (B) Expression of the truncated CKLiK (CKLiK296) but not the kinase dead form (CKLiK296-KD) inhibits the respiratory burst. Shown are the responses of two clonal sublines of each type of transduced EPRO cells as compared to cells expressing the empty vector. (C) Expression of the constitutively active CaMKKα (CaMKK413) inhibits the respiratory burst to levels similar to that observed by the constitutively active CKLiKs. Data shown for all assays are given as averages ± SD from triplicate samples analyzed in parallel and are representative of at least three independent experiments.