Fig. 2. Properties of rescued recombinant RVFV.

A) Plaque assay showing authentic ZH548 and the different recombinant viruses: rZH, rZHΔNSs, rZHΔNSs-GFP and rZHΔNSs-FLuc at passages 0 and 3. B) Growth curves of rZH and rZHΔNSs in comparison to ZH548 after infection of Vero cells at a moi=3. C) Expression of GFP in Vero cells infected with rZHΔNSs-GFP at passage 0 or 4 and in non-infected cells (NI). D) Kinetics of FLuc expression in Vero cells infected with rZHΔNSs-Fluc at a moi= 0.1 or 0.5. Luciferase activity is expressed in arbitrary units per 2.5×10⁴ cells. Each data point is the mean of triplicate assay.