Table 1.
Effects of T7- or Pol II- promoter driven protein expression plasmids on the efficiency of RVFV rescue using Pol I based plasmids for viral antigenome expression.
| Expression plasmid |
Titer* pfu/ml |
|||
|---|---|---|---|---|
| T7 promoter | Pol II promoter | |||
| pTM1-L (µg) | pTM1-N (µg) | pCAGGS-L (µg) | pCAGGS-N (µg) | |
| 0.25 | 0.25 | 4.0×107 | ||
| 0.5 | 0.5 | 8.8×107 | ||
| 1 | 1 | 7.0×107 | ||
| 0.5 | - | <10 | ||
| - | 0.5 | <10 | ||
| - | - | <10 | ||
| 0.25 | 0.25 | 1.4×107 | ||
| 0.5 | 0.5 | 2.4×107 | ||
| 1 | 1 | 1.7×107 | ||
| 0.25 | 0.5 | 2.2×107 | ||
| 0.25 | 1 | 2.9×107 | ||
| 0.5 | 1 | 1.6×107 | ||
| 0.5 | 0.25 | 5.0×107 | ||
| 1 | 0.5 | 1.6×107 | ||
| 1 | 5 | 1.4×107 | ||
*
Titer in pfu per ml in the maintenance medium collected at 5d post transfection