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Effect of various conditions for protein expression using the T7-promoter driven system for antigenome expression after transfection into BHK/T7-9 cells.
| Protein expression plasmids | Titer* | |
|---|---|---|
| pTM1-L (µg) | pTM1-N (µg) | pfu/ml |
| 0.5 | 0.5 | 1.7×107 |
| 0.5 | - | <10 |
| - | 0.5 | 8.0×106 |
| - | - | 7.2×107 |
titers in pfu per ml in the maintenance medium collected at 5d post transfection
