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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Mol Cell Neurosci. 2008 Apr 16;38(3):404–416. doi: 10.1016/j.mcn.2008.04.001

Figure 5.

Figure 5

(A) Expression of TrkB and ARMS expression is developmentally regulated. Whole cell lysates of primary cortical neurons were prepared from cultures incubated for various days in vitro (DIV) and immunoblot analyses were performed as indicated. (B) The extent of NF-κB activation induced by BDNF correlates with the expression of TrkB and ARMS. TransAM NF-κB DNA binding ELISAs were performed using nuclear extracts of primary cortical neurons treated with BDNF on different DIV. The data (expressed as relative light units) is the mean of three independent observations (bars indicate SEM). ### equals p<0.001. (C) Specificity of NF-κB ELISA. Nuclear extracts derived from neuronal cultures (DIV 9) treated with BDNF (100 ng/ml) were used for TransAM ELISA in the presence of 20× excess of oligonucleotides containing either WT or Mutant NF-κB consensus sequences. These assays demonstrate that the measured activity is indeed specific to NF-κB. ### equals p<0.001 as compared to ‘None’.