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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Cell Metab. 2008 Aug;8(2):105–117. doi: 10.1016/j.cmet.2008.06.013

Figure 2. Regulation of Fatty Acid β-oxidation by BAF60a in Primary Hepatocytes.

Figure 2

(A) Clustering analysis of gene expression in primary hepatocytes transduced with adenoviruses expressing GFP, PGC-1α, or individual TFs. Relative mRNA expression of genes involved in hepatic gluconeogenesis, fatty acid β-oxidation, mitochondrial OXPHOS, and circadian clock were analyzed using qPCR.

(B) qPCR analysis of gene expression in hepatocytes transduced with GFP (open) or BAF60a adenoviruses at multiplicity of infection of 1 (grey) or 3 (filled).

(C) qPCR analysis of relative mitochondrial/nuclear DNA content.

(D) Immunoblotting analysis of transduced primary hepatocytes.

(E–F) Relative rate of fatty acid oxidation (E) and secretion of TG-containing lipoproteins (F) in transduced hepatocytes. All data represent mean ± stdev. *p<0.05.