. Author manuscript; available in PMC: 2009 Sep 1.

Published in final edited form as: Biochim Biophys Acta. 2008 May 1;1777(9):1218–1228. doi: 10.1016/j.bbabio.2008.04.039

Table 4.

Effects of mutations on widths of b_H and b_L alpha bands and the positions of their resolved components.

Cyt b₅₆₁ mutant	Width at 75% Height (nm)^a		Shift in peak position, relative to wild type^b(nm)
Cyt b₅₆₁ mutant	b_H	b_L	b_H-1	b_H-2	b_L-1	b_L-2
Wild type	8.3	7.1	0	0	0	0
N78K	8.6	7.0	−0.2	+0.0	−0.1	+0.4
H92Q	6.1	7.0	+1.7	+0.8	+0.3	+0.4
H92Y	7.6	6.6	+0.7	+1.7	+0.0	+0.0
H109Y	8.8	7.1	−0.1	+0.1	−0.2	+0.0
H110Q	8.8	8.2	+0.1	+0.1	+0.3	+0.6
H110Y	8.4	7.5	+0.6	+0.9	+1.0	+1.0

From spectra in Fig. 6.

The positions of the major resolved bands in wild type cyt b₅₆₁ were: b_H-1, 561.0 nm; b_H-2, 554.6 nm; b_L-1, 560.8; b_L-2, 555.3 nm. Shifts were rounded to the nearest integer.