Table 3.
Strain | Redox Potentiometry Em, (V) | Nernst Equation Solution Fitted at Each Wavelength of the Spectrum, Em, (V) | ||||||
---|---|---|---|---|---|---|---|---|
bL | bH | b150 | cyt c1 | bL | bH | b150 | cyt c1 | |
WT + antimycin | −0.113 | 0.049 | --- | 0.250 | −0.090 | 0.040 | --- | 0.250 |
WT | −0.120 | 0.054 | 0.169 | 0.250 | −0.090 | 0.040 | 0.150 | 0.250 |
Y199L | −0.123 | 0.038 | 0.149 | 0.259 | −0.100 | 0.030 | 0.148 | 0.250 |
Y199S | −0.077 | 0.057 | 0.172 | 0.265 | −0.094 | 0.053 | 0.170 | 0.260 |
Y199T | −0.108 | 0.045 | 0.160 | 0.262 | −0.092 | 0.027 | 0.152 | 0.250 |
199-insert | −0.096 | 0.035 | 0.155 | 0.251 | −0.092 | 0.033 | 0.135 | 0.250 |
Y199W | −0.080 | 0.075 | 0.170 | 0.237 | −0.091 | 0.050 | 0.150 | 0.250 |