TABLE 3.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| E. coli | ||
| DH5 αMCR | F−mcrAΔ 1 (mrr-hsd RMS-mcrBC) φ80dlacZΔ M15 Δ (lacZYA-argF)U169 deoR recA1 endA1 supE44 λ thi-1 gyr-496 relA1 | GIBCO-BRL |
| B. subtilis | ||
| BR151MA | lys3 trpC2 (wild type) | Grundy et al. (1994) |
| Δ PS | BR151MA Δ motPS::neo | Ito et al., 2004 |
| Δ ABΔ PS | BR151MA Δ motPS::neo Δ motAB::SpcR | This study |
| MotAB | Δ ABΔ PS amyE::PmotAB-motAB | This study |
| MotAB-Spac | Δ ABΔ PS amyE::Pspac-motAB | This study |
| MotPS | Δ ABΔ PS amyE::PmotAB-motPS | This study |
| MotPS-Spac | Δ ABΔ PS amyE::Pspac-motPS | This study |
| MotAS | Δ ABΔ PS amyE::PmotAB-motAS | This study |
| MotAS-Spac | Δ ABΔ PS amyE::PspacmotAS | This study |
| MotAS-M | Δ ABΔ PS amyE::PspacmotAS, up-motile variant | This study |
| MotPB | Δ ABΔ PS amyE::PmotAB-motPB | This study |
| MotPB-Spac | Δ ABΔ PS amyE::Pspac-motPB | This study |
| MotPB-M | Δ ABΔ PS amyE::Pspac-motPB, up-motile variant | This study |
| Plasmids | ||
| pMW118 | Cloning vector (Apr) | Nippon Gene |
| pDR67 | amyE integration vector with Cmr gene and Pspac promoter upstream of multiple cloning site | Ireton, K., et al. 1993. |
| pDG1730 | amyE integration vector with Ampr, Specr, and Ermr genes | Guerout-Fleury et al. 1996 |
| pDR-AB | pDR67 + motAB | This study |
| pDR-AS | pDR67 + motAS | This study |
| pDR-PS | pDR67 + motPS | This study |
| pDR-PB | pDR67 + motPB | This study |
| pDG-AB | pDG1730 + PmotAB-motAB | This study |
| pDG-AS | pDG1730 + PmotAB-motAS | This study |
| pDG-PS | pDG1730 + PmotAB-motPS | This study |
| pDG-PB | pDG1730 + PmotAB-motPB | This study |