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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Cell Signal. 2008 May 18;20(9):1632–1641. doi: 10.1016/j.cellsig.2008.05.003

Fig. 3.

Fig. 3

Physical interaction between Smad2 and Tcf4. (A) GST-fused Smad2 deletion constructs. (B) In vitro binding (GST-pull down) assay was performed between bacterially expressed GST-fused full-length Smad2 protein and in vitro translated Myc-tagged full-length Tcf4 (Myc-Tcf4) (left panel) and between GST-fused Smad2MH2 (271–467) domain and in vitro translated Myc-Tcf4 (right panel). Protein interaction was visualized by Western blot analysis with anti-Myc tag antibody. (C) Myc-tagged Tcf4 deletion constructs. (D) GST-pull down assays were performed using GST-fused Smad2MH2 domain (Smad2MH2) and Myc-Tcf4 or indicated Tcf4 deletion constructs.