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Nutritional regulation of SIRT1 protein and activity levels in the brain of C57BL/6 mice. SIRT1, acetylated lysine (K) 379 in p53 and β-actin (used as loading control) protein levels were assessed in the hypothalamus, the forebrain (without the hypothalamus), and the hindbrain between the fed and fasted states by Western blot analysis. Western blot data represent the results of 5–10 samples.
