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Nutritional regulation of SIRT1 protein and activity levels in the brain of obese, ob/ob C57BL/6 mice. SIRT1, acetylated lysine (K) 379 in p53 and β-actin (used as loading control) protein levels were assessed in the hypothalamus, the forebrain (without the hypothalamus), and the hindbrain of obese mice between the fed and fasted statuses by Western blot analysis.
