FIGURE 10.

Analyses of CFM3 function in mitochondria. (A) Immunoblots of total leaf extract were probed with antibodies to cytochrome c1 or mitochondrial Rps12. The corresponding Ponceau S stained blot is shown in the bottom panel of Figure 5. (B) RNA gel blot analysis of total leaf RNA (0.5 μg), using probes from the mitochondrial rrn18 and rrn26 genes. The forms marked as “mature” are based on the annotated termini of these rRNAs. The methylene blue-stained blot is shown below. (C) Sucrose-gradient fractionation of maize mitochondrial extract. Maize mitochondria were solubilized with 1% NP-40 and sedimented through a sucrose gradient under conditions in which particles greater than ∼70S pellet. An equal portion of each fraction was analyzed on immunoblots, by probing with CFM3 or RPS12 antiserum. RNA purified from the same fractions was analyzed by RNA gel blot hybridization, using a probe for rrn18 (bottom panel).