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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Free Radic Biol Med. 2008 Jun 27;45(7):994–1001. doi: 10.1016/j.freeradbiomed.2008.06.017

Table 1.

Direct quantification of BH4, BH2 and ascorbate in adult rat cardiomyocytes by HPLC with electrochemical detection.

Treatment BH4 (pmol/mg protein) BH2 (pmol/mg protein) Ascorbate (pmol/mg protein)
Control 0.066±0.058 0.0 13.80±2.10
TNF-α 0.039±0.058 0.0 13.23±0.14
IFN-γ 0.057±0.035 0.0 13.83±1.92
IL-1 0.077±0.087 0.0 12.51±1.57
TNF-α + I FN-γ 0.078±0.093 0.0 20.68±1.40

Adult cardiomyocytes were treated with cytokines 12 hr as follows: TNF-α (20 ng/ml); IFN-γ (50 ng/ml); IL-1 (20 ng/ml). Values represent the mean±SD of at least 3 experiments.

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