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. Author manuscript; available in PMC: 2009 Sep 15.
Published in final edited form as: Free Radic Biol Med. 2008 Jun 14;45(6):773–779. doi: 10.1016/j.freeradbiomed.2008.05.030

Figure 5.

Figure 5

Immunopreciptitation (A) and oxidation (B) of DJ-1 in s-IBM and Control muscle biopsies. A- immunoprecipitation (IP) was performed using an anti-DJ-1 rabbit polyclonal antibody, followed by immunoblotting (IB) using an anti-DJ-1 mouse monoclonal antibody. #, the primary antibody was omitted from the immunoprecipitation reaction in order to ascertain the specificity of the reaction. B-The oxyblot, demonstrating protein carbonyl content, as an indicator of oxidation of the protein, shows that DJ-1 is highly oxidized in s-IBM muscle fibers, but not in age-matched Control muscles (details in text).