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. Author manuscript; available in PMC: 2009 Jul 15.
Published in final edited form as: Bioorg Med Chem. 2008 Jun 5;16(14):6696–6701. doi: 10.1016/j.bmc.2008.05.078

Figure 2. The GLA derivative 17 inhibited the chymotrypsin-like activity of the proteasome in MT4 cells.

Figure 2

A Promega cell-based proteasome assay was used to determine the effect of compound 17 on the ChT-L activity of 20S proteasome (13). The known proteasome inhibitors LLM-f and lactacystin were used as positive controls for proteasome inhibition. MT4 cells are human T cells isolated from a patient with adult T-cell leukemia. The ChT-L proteasome activity was detected as the relative light unit (RLU) generated from the cleaved substrate in the assay. The % control is derived using the formula: 100 × (RLU with test compound) / (RLU from the control without compound).