TBP I: inside (“nested”) primers for nested amplification
sense primer	5′–gga cca gaa caa cag cct tc–3′ pos 3
anti-sense primer	5′–ccg taa ggc atc att gga ct–3′ pos 104
probe	5′–FAM cat gac tcc tgg aat tcc cat ctt t–TAMRA–3′