Table 1.
Inhibitor | Ki for Vacc TMP kinase | Ki for human TMP kinase, mM |
---|---|---|
dT | 25 ± 5 μM | 0.25 |
dU | 1.0 ± 0.1 mM | 2.5 |
5F-dU | 0.7 ± 0.1 mM | >5 |
5Br-dU | 7 ± 1 μM | 0.2 |
5Cl-dU | 15 ± 5 μM | 0.37 |
5I-dU | 23 ± 2 μM | 0.35 |
BVdU | 0.5 ± 0.1 mM | 1.1 ± 0.1 |
5I-BrV-dU | 0.5 ± 0.05 mM | 0.3 ± 0.05 |
5diIV-dU | 0.6 ± 0.1 mM | 0.35 ± 0.15 |
Activities were measured in standard conditions with [ATP] = 1 mM, [TMP] from 10 μM to 1 mM, and varying inhibitor concentrations (0-5-50 μM for the more efficient). KI values were obtained from double reciprocal plots. Shown in italics are data from ref. 41.