Fig. 5.

Rad51 can use dsDNA processed by hExo1 and BLM for homologous DNA pairing. Joint molecule reactions were performed as described in Materials and Methods. (A) Schematic representation of the reaction. The asterisks indicate ³²P label on the 3′ ends. (B) Joint molecule formation requires hRad51. Gel shows reaction products in the absence of hRPA. Lanes: 1, absence of proteins; 2, BLM and hExo1; 3, BLM, hExo1, and hRad51. (C) Joint molecule formation requires hExo1. Lanes 1 and 2, joint molecule reactions in the absence and presence of hExo1, respectively. (D) Joint molecule formation as a function of BLM concentration. Lane 1, absence of proteins; 2–5, increasing amounts of BLM (0, 20, 40, 80 nM). The positions of intact DNA (2.7 kbp), resection products, joint molecules, and molecular size standards (kbp) are indicated.