Fig. 3.

Genetic and pharmacologic ablation of IDO blocks induction of pDC suppressor activity. PMA was applied to skin of IDO1-KO (A); B6 (WT) mice (B–D). Some mice were also given (C) IDO inhibitor (D-1MT, 2 mg/mL) and sweetener or (D) sweetener only in drinking water starting 2 days before the first PMA treatment (day 0) until 2 days after the third PMA treatment (day 9). As before, sorted LN pDCs were incubated with OT-1 responders and OVA peptide with or without D-1MT. Data are representative of at least 2 experiments. Data shows significant suppression (B and D, P < 0.05) or no significant suppression (A and C, P > 0.05) and triplicate (or more) cultures were analyzed.