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. 2008 Oct 28;105(44):17091–17096. doi: 10.1073/pnas.0809288105

Fig. 2.

Fig. 2.

IKKα down-regulation in primary keratinocytes interferes with expression of TGF-β-regulated genes. (A) Total RNA from control or IKKα-silenced primary keratinocytes was analyzed using Mouse Genome 420 microarrays. Data were analyzed by dChip software (Department of Biostatistics, Dana Farber Institute, Harvard, MA). The heatmap of genes belonging to the TGF-β pathway that were significantly up- or down-regulated in IKKα-silenced cells is shown. (B) Primary mouse keratinocytes were transfected with IKKα-specific or control siRNA, and expression of IKKα, Ovol1, Ovol2, Mad1, Mad2, Mad3, and Mad4 mRNAs was examined 24 h after transfection by quantitative RT-qPCR. The results shown are representative of 3 independent experiments. Statistical significance is indicated. Histogram is representative of 3 experiments performed in triplicate. Error bars represent 1 standard deviation.