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. 2008 Oct 24;105(44):17103–17108. doi: 10.1073/pnas.0802696105

Fig. 3.

Fig. 3.

Deletion of NHR4 or C663S mutation abolishes SON interaction but not N-CoR interaction. (A) Effects of the C663S mutation on interactions with the SON N-terminal fragment and the N-CoR fragment containing the RIII domain. GST-tagged SON (1–81 a.a.) and HA-tagged N-CoR (975–1250 a.a.) were transfected into 293T cells with either Flag-tagged wild-type ETO (WT) or ETO with C663S mutation, and immunoprecipited with Flag antibody. (B) Effects of NHR4 deletion or C663S mutation on interactions with full-length SON and N-CoR. SON antibody or N-CoR antibody was used for immunoprecipitation to pull down endogenous SON or N-CoR, and immunoblotted to detect Flag-tagged ETO; wild-type ETO (WT), ETO with a C663S mutation (C663S), and NHR4-deleted ETO (ΔNHR4).