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. 2008 Oct 24;105(44):17103–17108. doi: 10.1073/pnas.0802696105

Fig. 5.

Fig. 5.

Expression of SON N-terminal fragment inhibits AML1-ETO binding to endogenous SON, and rescues cells from AML1-ETO-induced growth arrest. (A) The SON N-terminal fragment encoding a.a. 1–140 (Flag-SON-140) colocalizes with endogenous SON. K562 cells expressing MigR1-Flag-SON-140 were immunostained with SON antibody (recognizing C terminus of SON) for endogenous SON, and with Flag antibody for the SON-140 fragment. (B) Expression of the SON-140 fragment interferes with the interaction between endogenous SON and AML1-ETO. K562 cells stably expressing control vector or Flag-SON-140 were transfected with HA-tagged AML1-ETO, and immunoprecipitation was performed with SON antibody, and the immunocomplexes were subjected to Western blotting with the indicated antibodies. (C) Expression of the SON-140 fragment alone does not affect cell growth and rescues cells from AML1-ETO-induced growth arrest. U937T cells with inducible AML1-ETO expression were infected with control vector or Flag-SON-140. The GFP-positive populations were sorted, cultured with or without tetracycline, and cells were counted every other day. Western blot shows expression of Flag-tagged SON-140 in sorted cells. Growth curve shown represents four independent experiments. Each circle and error bar represents mean ± SD, from the triplicate cell culture.