Fig. 2.

CREB2 binds importin α1 and 6. (A) FLAG-tagged importin α isoforms expressed in 293T cells were incubated with GST-CREB2 beads, and immunoblotted with anti-FLAG antibody. GST-CREB2 pulled down importin α1 and 6, but not α2, 3, or 4. (B) Mutations in the NLS of CREB2 (CREB2mNLS) reduced the amount of interacting importin α1. Immunoblotting with GST antibodies (A and B Lower) shows that equal amounts of GST-CREB2 were used in each pull-down assay (note that GST alone runs at a lower MW and is not shown).