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. 2008 Oct 28;105(44):17175–17180. doi: 10.1073/pnas.0803906105

Fig. 6.

Fig. 6.

ApCREB2 accumulates in the nucleus of SNs during FMFRamide-induced LTD of sensory-motor synapses. SN-MN cultures (5DIV) received 5 spaced applications of FMFRamide (5xFMFRa) to induce LTD (A) or 5 spaced applications of 5HT (5x5HT) to induce long-term facilitation (B), or 5 applications of ASW (5xASW) as control. Cultures were immediately fixed and processed for immunocytochemistry with anti-ApCREB2 antibodies. Representative confocal optical sections through the nucleus are shown on the left and group data on the right. (A) Spaced application of FMFRamide increased the concentration of ApCREB2 in the SN nucleus but not in the MN nucleus, whereas ASW had no effect (*, P < 0.05, ASW SN, n = 23 vs. FMFRamide SN, n = 19, unpaired t test, t = 2.2, df = 40). (Scale bar, 40 μm.) (B) Spaced application of 5HT did not significantly alter the nuclear concentration of CREB2 in either SN or MN (n = 11 for ASW, and 7 for 5HT for both SN and MN).