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. 2008 Oct 28;105(44):17187–17192. doi: 10.1073/pnas.0808207105

Fig. 4.

Fig. 4.

SRC-3 promotes GCN5 expression (A) GCN5 mRNA level in gastrocnemius muscle and brown adipose tissue (BAT) of SRC-3+/+ and SRC-3−/− mice fed a high fat diet (n = 8). (B) GCN5 mRNA level in C2C12 cells infected with an adenovirus expressing SRC-3 (C) GCN5 mRNA level in C2C12 cells transfected with either a control siRNA or a SRC-3 siRNA (n = 6). (D) ChIP assay showing the specific recruitment of SRC-3 on the promoter but not on the coding or 3′UTR of the mGCN5 gene. (E) Luciferase activity detected in extracts of C2C12 cells transfected with a GCN5 promoter luciferase reporter construct and cotransfected or not with SRC-3 (n = 6). (F) Acetylation level of PGC-1α determined from total extracts of C2C12 cells infected with a combination of adenoviruses, expressing PGC-1α, GCN5 and/or SRC-3. Western-blot analysis of SRC-3 and GCN5 are shown in the bottom panels, whereas the levels of acetylated (Ac-Lys) and total PGC-1α are depicted in the top panels. *, P < 0.05 and **, P < 0.005 are indicative of significant differences.