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Sequence specificity of 5-MCDG. Enzymatic reactions were carried out as described in Materials and Methods. Upon alkaline treatment the reaction product was separated on a 20% polyacrylamide DNA sequencing gel. a is the labeled oligonucleotide incubated with the enzyme, and b is the same oligonucleotide incubated without the enzyme. The arrowhead shows the position of the cleavage product. NM, nonmethylated DNA strand; M, methylated strand.
