Figure 3.

Construction of deletion mutants of chicken G/T mismatch DNA glycosylase. The constructs were done as described in Materials and Methods. (A) The relative activities of G/T mismatch and 5-MeC glycosylase are indicated by + or −. The radioactive bands corresponding to the specific reaction product were cut out from the gels and counted for radioactivity. The ratio of the two enzyme activities is given. (B) A silver-stained gel of mutant enzyme isolated on a Ni-nitrilotriacetic acid-agarose column.