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Potentiation of 5-MCDG by RNA helicase, RNA, and ATP. Incubations were assembled in duplicates and carried out as described in Materials and Methods. Bands corresponding to the specific reaction products were cut out from the gel and counted for radioactivity. Blanks were subtracted from the test values and expressed in cpm/μg enzyme. The value obtained for the enzyme incubated without any further addition was expressed as 100%.
