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Estimate of dG content in moth DNA. All moth samples were subjected to LC-MS analysis. For the software analysis, ions of the four deoxynucleosides were extracted from the total ion currents and resulting peaks were integrated. The plot shows the ratios of integrals for dA/dT and dG/dC, respectively. While the dA/dT ratio stays fairly constant over time, the dG/dC ratio drops very much. Indeed, dG cannot be detected in the oldest moth samples.
