Table 1.
The kinetics of coexpression of TH components and marker genes at Stage 31
gene 1 | gene 2 | % gene 1+ | % gene 2+ | % gene 1+/gene 2+* | % gene 2+/gene 1+ | % [3H]+ | % of gene 1+ cells that are [3H]+ | % of gene 2+ cells that are [3H]+ | % of [3H]+ cells that are gene 1+ | % of [3H]+ cells that are gene 2+ | % of [3H]+ cells that are both gene 1+ and gene 2+ |
otx2 | TRb | 36.4 | 15.2 | 41 | 97.8 | 14.9 | 9.9 | 2.2 | 24.2 | 2.2 | 2.2 |
dio2 | cath5 | 1.2 | 10.2 | 30 | 3.4 | 17.5 | 0 | 1.1 | 0 | 0.7 | 0 |
cath5 | neurod | 16.5 | 28.3 | 28.4 | 16.5 | 15 | 2.5 | 13 | 2.7 | 24.3 | 2.7 |
Cells were labeled in ovo with [3H]-thymidine for 1 hour, and tissue was harvested and dissociated at the indicated times. Double DISH for the indicated genes and autoradiography for [3H] were carried out on the dissociated cells. Cells were quantified for labeling by a single gene probe, both gene probes, and labeling with [3H].