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. 2008 Nov 14;3(11):e3730. doi: 10.1371/journal.pone.0003730

Figure 1. uPAR is present in early endosomes and late endosomes/lysosomes in the presence of endogenous ligands (HT1080 cells).

Figure 1

Immunofluorescence of HT1080 cells (left panel) co-stained with anti-uPAR antibody (green) and monoclonal antibody anti-EEA1 (red, top) and monoclonal antibody anti-LAMP1 (red, bottom), respectively, and visualized with confocal laser microscopy. Overlays (merge) show the co-localization of uPAR with EEA1 and LAMP-1 positive membranes (yellow). Scale bars 10 µm. On the right side is shown a representative electron micrograph of uPAR (protein A gold 10 nm) present in early endocytic (full arrow) and lysosomal (arrowhead) compartments previously loaded with BSA 5 nm gold for 2 hours as fluid phase tracer. Scale bar 200 nm.