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Panel A: Biotinylation experiments in HEK293-uPAR cells incubated in the absence or presence of Wortmannin and RAP. Lane 1: no treatment, Lane 2: 200 nM RAP, Lane 3: 300 nM Wortmannin, Lane 4: Wortmannin and RAP. Wortmannin and RAP do not inhibit constitutive uPAR endocytosis after 15 min at 37°C. Panel B: densitometric analysis of the immunoblots of Panel A.
