Skip to main content
. 2008 Jul 29;93(10):4065–4074. doi: 10.1210/jc.2008-0396

Figure 1.

Figure 1

Responsiveness of human islets to glucose-induced IL-1β expression and IL-1Ra inhibition depends on baseline IL-1β expression levels. Human islets were cultured for 4 d at 5.5 (basal) or 33.3 mm glucose. IL-1β and IL-1Ra mRNA levels were quantified using 18s RNA as an internal standard. The mean of triplicate determinations for each preparation is shown. A, Basal IL-1β mRNA levels expressed relative to a human islet preparation arbitrarily designated as H0 and defined as 1. B Effect of 33.3 vs. 5.5 mm glucose on IL-1β mRNA in different human islet preparations expressed as fold of basal levels (5.5 mm glucose). C, Effect of 33.3 mm glucose on IL-1Ra mRNA expressed as fold of basal levels, D, Variation of basal expression of different gene products in 11–12 human islet preparations. For each separate gene transcript, we defined the sample with the lowest expression as 1. E, Linear regression analysis of basal IL-1β mRNA vs. fold stimulation of IL-1β mRNA by glucose. F, Fold glucose-stimulated IL1β mRNA vs. fold glucose-inhibited IL-1Ra mRNA.