Table 2.

Percentages of CpG methylation within the IG-DMR in normal pituitaries and pituitary tumors

	CpG1	CpG2	CpG3	CpG4	CpG5	CpG6	CpG7	CpG8
Secretory tumors
GH-secreting
1	65	95	85	60	60	90	45	70
2	65	55	70	75	90	70	85	70
3	35	85	90	55	35	75	65	50
PRL-secreting
1	100	45	100	100	100	40	35	35
2	95	50	100	100	100	100	45	45
3	40	60	65	55	40	60	55	45
ACTH-secreting
1	60	70	70	70	80	25	60	30
2	80	100	95	85	95	90	55	55
3	75	60	75	70	90	45	40	35
4	70	90	95	75	85	70	70	80
Mean	69%	71%	85%	75%	78%	67%	56%	52%
NFT
1	50	100	100	66	66	66	75	50
2	40	50	86	95	60	66	95	95
3	57	87	74	57	87	57	61	74
4	33	86	95	57	48	81	33	24
5	33	50	83	67	50	58	100	83
6	100	68	95	77	59	65	100	100
7	57	48	57	57	95	43	100	48
8	90	60	100	40	90	40	40	40
9	60	90	70	70	70	70	80	50
10	94	65	100	82	76	76	65	59
11	70	76	100	88	82	47	88	82
Mean	62%	71%	87%	69%	71%	61%	76%	64%
Normal
1	43	56	75	50	56	68	55	50
2	44	44	75	56	37	44	37.5	62.5
3	31	56	75	56	62	25	60	37
4	44	44	75	56	62.5	50	62.5	68
5	31	56	75	56	68	25	50	37.5
Mean	39%	51%	75%	55%	57%	42%	53%	51%

Each number indicates the percentage of methylation at each CpG dinucleotide position with the IG-DMR among 20 clones analyzed. Normal indicates normal human pituitaries. NFT, Clinically nonfunctioning pituitary adenomas.