Figure 1.

Verification of the Nb2 bioassay specificity. Left, Nb2 cell proliferation in response to hPRL and hGH. Rat Nb2 lymphocytes were incubated with increasing amounts of hPRL or hGH. After 3 d, cell proliferation was measured by the MTT method. Right, Confirmation of PRL identity in CM as determined by immunoneutralization. Aliquots of CM from adipose explants, hPRL (50 pg/well), and hGH (50 pg/well) were incubated with Nb2 cells in the presence of rabbit IgG (control, 5 μg/well), anti-hPRL IgG (5 μg/well), or anti-hGH antiserum (1:200).