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. 2008 Jul 21;155(4):505–513. doi: 10.1038/bjp.2008.292

Figure 5.

Figure 5

YC-1-induced apoptosis is mediated through JNK pathway. A498 cells were incubated in the absence or presence of YC-1 (0.3 μM) for the times indicated, and cells harvested and prepared for the detection of phospho-JNK and internal control α-tubulin expression using western blot analysis. (b) Cells were preincubated in the absence or presence of SP600125 for 1 h, and then YC-1 was added to the cells for 24 h. The cytotoxic effect was determined using MTT assay method as described in Materials and methods section. **P<0.01 as compared with the control group, #P<0.05 as compared with the YC-1-treated group. Cells were preincubated in the absence or presence of SP600125 for 1 h, and then YC-1 was added to the cells for 24 h. The activation of phosphorylation of JNK (c) and caspase 8 (d) were detected by western blot analysis. (e) A498 cells were transfected with JNK1/2 siRNA and placed in 96-well plates 3 days after transfection. Cell viability, phospho-JNK, total JNK and caspase 8 were assayed as described in Materials and methods section. Data are the mean±s.e.mean of three independent experiments. #P<0.001 and **P<0.01 versus YC-1-treated groups. JNK, Jun N-terminal kinase; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.