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. 2008 Oct 14;99(9):1493–1501. doi: 10.1038/sj.bjc.6604727

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Copyright © 2008 Cancer Research UK

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(A) Effects of intrakines or RNAi on CXCR4 and CXCR7 surface levels of KEP1 mammary carcinoma cells. Open histograms: negative controls (second antibody only for CXCR4 or preimmune serum and second antibody for CXCR7). Grey filled histograms: either CXCR4 mAb (upper row) or CXCR7 antiserum (lower row). (B) Effect of intrakines and RNAi on proliferation of KEP1 cells in 1% FCS-containing supernatants, as in Figure 1. Concentrations of both CXCL12 and mutant were saturating, as the effects were similar at 50% dilution (not shown). Results are from one of the two experiments with virtually identical results.