Figure 1.

(A) Schematic diagram for rAdCMVAK gene construct. Expression was driven by nonspecific CMV promoter and was stabilized by an simian virus 40 polyadenylation sequence (SV40pA). From this construct, a ΔE1–ΔE3 adenovirus was prepared by using published methods (17). (B) RT-PCR was used to detect the presence of AK transcripts in rAdCMVAK-injected muscles. Total RNA isolated from frozen tissue was subjected to RT-PCR by using oligonucleotides specific for Drosophila AK and glyceraldehyde 3-phosphate dehydrogenase (GAPDH). AK transcripts were detected in rAdCMVAK-injected muscles (+AdAK) but not in the contralateral control (No Inj). AK and GAPDH primers served as positive controls for the procedure (Pos Ctrl).