Table 1.
Mosaic analysis of yan and pointed mutants.
| A | |||||
|---|---|---|---|---|---|
| Allele | R3 mutant | R4 mutant | R2 mutant | R5 mutant | R8 mutant |
| yanE433 | |||||
| wild-type
(m∂ in R4) |
11 | 3 | 1 | ||
| mutant
(m∂ in R3+R4) |
4 | 1 | |||
| n | 4 | 11 | 3 | 1 | 1 |
| yanXE18 | |||||
| wild-type
(m∂ in R4) |
11 | 17 | 5 | 3 | |
| mutant
(m∂ in R3+R4) |
12 | ||||
| n | 23 | 17 | 5 | 3 | 0 |
| total n | 27 | 28 | 8 | 4 | 1 |
| effect/conclusion | yan represses R4 fate in R3 | yan function is dispensable in R4 | under represented genotype | under represented genotype | under represented genotype |
| B | |||||
|
Allele
Marker |
R3 mutant | R4 mutant | R2 mutant | R5 mutant | R8 mutant |
|
pnt1230
psqGAL4, UAS- GFP |
|||||
| wild-type
(R3 high, R4 low) |
3 | 5 | 1 | 1 | |
| mutant
(symmetrical) (inverted) |
3
1 |
1 | 1 | ||
| n | 7 | 6 | 2 | 1 | 0 |
|
pnt19099
m∂-lacZ |
|||||
| wild-type
(m∂ in R4) |
1 | 3 | |||
| mutant
(symmetrical) (inverted) |
1 | 1 | 2
1 |
2 | 1 |
| n | 2 | 1 | 3 | 2 | 4 |
|
pnt198
m∂-lacZ |
|||||
| wild-type
(m∂ in R4) |
4 | ||||
| mutant
(symmetrical) (inverted) |
2
1 |
2 | 3 | 1 | |
| n | 3 | 2 | 0 | 3 | 5 |
| total n | 12 | 9 | 5 | 6 | 9 |
| wild-type | 4 | 5 | 1 | 1 | 7 |
| mutant
(symmetrical) (inverted) |
5
3 |
4 | 3
1 |
5 | 2 |
| effect | loss of R4 fate or inverted R3/R4 fates | loss of R4 fate | loss of R4 fate or inverted R3/R4 fates | loss of R4 fate | loss of R4 fate |
Summary of single mutant cell analysis in 5 cell preclusters in developing mosaic eyes. R3, R4, R2, R5 and R8 were scored for loss of gene function and R4 fate was monitored with m∂-lacZ. (A) For yanE433 and yanXE18 mosaic clusters were counted in yw eyFLP; yan- FRT40/ ubiGFP FRT40A; m∂-lacZ/+ eyes. If yan was mutant in the R3 cell 59.3% of these cells adopted the R4 fate (n=27). No effect was observed in R4 (n=28). Clones for R2, R5 and R8 were underrepresented due to the general function of yan as a repressor of differentiation and the sequential recruitment of R-cells to the cluster.
(B) For pnt1230, pnt19099 and pnt198 mosaic clusters were counted as described above. For all 3 alleles single cell clones for R2, R5, R3 and R4 abolished the R4 cell fate and/or changed the R3 fate to R4. Clones for R8 showed mostly no effect.