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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Dev Biol. 2008 Jan 26;316(1):110–123. doi: 10.1016/j.ydbio.2008.01.016

Table 1.

Mosaic analysis of yan and pointed mutants.

A
Allele R3 mutant R4 mutant R2 mutant R5 mutant R8 mutant
yanE433
wild-type
(m∂ in R4)
11 3 1
mutant
(m in R3+R4)
4 1
n 4 11 3 1 1
yanXE18
wild-type
(m∂ in R4)
11 17 5 3
mutant
(m∂ in R3+R4)
12
n 23 17 5 3 0
total n 27 28 8 4 1
effect/conclusion yan represses R4 fate in R3 yan function is dispensable in R4 under represented genotype under represented genotype under represented genotype
B
Allele
Marker
R3 mutant R4 mutant R2 mutant R5 mutant R8 mutant
pnt1230
psqGAL4, UAS- GFP
wild-type
(R3 high, R4 low)
3 5 1 1
mutant
(symmetrical)
(inverted)
3
1
1 1
n 7 6 2 1 0
pnt19099
m∂-lacZ
wild-type
(m∂ in R4)
1 3
mutant
(symmetrical)
(inverted)
1 1 2
1
2 1
n 2 1 3 2 4
pnt198
m∂-lacZ
wild-type
(m∂ in R4)
4
mutant
(symmetrical)
(inverted)
2
1
2 3 1
n 3 2 0 3 5
total n 12 9 5 6 9
wild-type 4 5 1 1 7
mutant
(symmetrical)
(inverted)
5
3
4 3
1
5 2
effect loss of R4 fate or inverted R3/R4 fates loss of R4 fate loss of R4 fate or inverted R3/R4 fates loss of R4 fate loss of R4 fate

Summary of single mutant cell analysis in 5 cell preclusters in developing mosaic eyes. R3, R4, R2, R5 and R8 were scored for loss of gene function and R4 fate was monitored with m∂-lacZ. (A) For yanE433 and yanXE18 mosaic clusters were counted in yw eyFLP; yan- FRT40/ ubiGFP FRT40A; m∂-lacZ/+ eyes. If yan was mutant in the R3 cell 59.3% of these cells adopted the R4 fate (n=27). No effect was observed in R4 (n=28). Clones for R2, R5 and R8 were underrepresented due to the general function of yan as a repressor of differentiation and the sequential recruitment of R-cells to the cluster.

(B) For pnt1230, pnt19099 and pnt198 mosaic clusters were counted as described above. For all 3 alleles single cell clones for R2, R5, R3 and R4 abolished the R4 cell fate and/or changed the R3 fate to R4. Clones for R8 showed mostly no effect.

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