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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: Biochim Biophys Acta. 2008 Jun 6;1784(9):1301–1309. doi: 10.1016/j.bbapap.2008.05.010

Figure 6. Proteolysis gels.

Figure 6

The target proteins were analyzed for resistance to PK by SDS-PAGE as described. Each assay consists of 2.5 µg protein and some geometric PK dilution series, with the most concentrated PK to the left as indicated by the triangles above the gels. Standard experiments used a range of 68 to .068 ng and are indicated by larger black triangles. Two proteins, D7:8 and D8:9, exhibited extreme sensitivity, and a more dilute series was also run, PK range from 2.1 to 0.002 ng. These are indicated by the smaller white triangles. This dilution series is such that a 10-fold reduction in PK is achieved in two lanes, and the 31.6 dilution between the regular and low concentration series is such that the left most lane of the diluted series corresponds to the middle lane of the regular series. Each gel also has a standards lane, STD, with markers of 14, 27 and 40 kDa.