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. Author manuscript; available in PMC: 2008 Nov 9.
Published in final edited form as: Mol Cell. 2008 May 9;30(3):277–289. doi: 10.1016/j.molcel.2008.03.016

Figure 2. p53 affects Chk2-P levels and dynamics.

Figure 2

(A) Immunoblots of Chk2-P(T68) in MCF7 cells transfected with control or p53 siRNA 48h prior to harvesting. Cells were harvested before irradiation or 1h after irradiation with 10 Gy of γ-irradiation. (B) Immunoblots of Chk2-P(T68) and p53 kinetics in MCF7 cells transfected with p53 siRNA 48h prior to irradiation with 10 Gy of γ -irradiation. Cells were harvested at the indicated times after irradiation. The graph shows quantification of Chk2-P levels normalized by tubulin and relative to peak intensity. Blots are representative of triplicate experiments. (C, D) Immunoblots of Chk2-P(T68) and p53 kinetics in MCF7 cells treated with (C) 5 µM Nutlin3 for 2h or (D) 10 µM Nutlin3 for 4h, prior to irradiation with 10 Gy of γ-irradiation. Cells were harvested at the indicated time after irradiation. Blots are representative of duplicate experiments.