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Identified FOXO3 variants do not affect ability to transactivate reporter gene transcription. NIH3T3 cells were transiently co-transfected with FHRE-luc reporter construct (Brunet et al., 1999), a tk-Renilla luciferase reporter and the appropriate control or experimental FOXO3 cDNA. The cells were incubated for 36 hrs and luciferase activity assayed.
