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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Am J Physiol Heart Circ Physiol. 2008 Apr 25;294(6):H2814–H2821. doi: 10.1152/ajpheart.00095.2008

Fig. 1.

Fig. 1

ANG II blocks serum deprivation-induced apoptosis in microvascular endothelial cells (ECs) through the angiotensin type 1 (AT1) receptor. Cells were treated with either serum-free medium or serum free + ANG II (1–100 nmol/l). A: representative fluorescence images of terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assays from cells in RPMI medium and 20% serum (CT), RPMI serum free (SF), and serum-free medium plus 50 nmol ANG II (SF + ANG II 50 nmol). Apoptotic cells are shown in yellow. Magnification, ×20. B: total number of cells stained by 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) after 24 h incubation period. C: dose-response analysis of the antiapoptotic effects of ANG II after 24 h incubation period. Values represent means ± SE. Each point is the mean of data from duplicate experiments. *P < 0.05 vs. CT; +P < 0.05 vs. SF; †P < 0.05 vs. respective ANG II dose.