Skip to main content
Infection and Immunity logoLink to Infection and Immunity
. 1991 Jun;59(6):2186–2188. doi: 10.1128/iai.59.6.2186-2188.1991

Purification and characterization of a new heat-stable enterotoxin produced by Vibrio cholerae non-O1 serogroup Hakata.

M Arita 1, T Honda 1, T Miwatani 1, K Ohmori 1, T Takao 1, Y Shimonishi 1
PMCID: PMC257985  PMID: 2037379

Abstract

The possible production of a heat-stable enterotoxin (Vc-H-ST) by Vibrio cholerae non-O1 serogroup Hakata was investigated, and the purified Vc-H-ST was characterized. It has a unique amino acid sequence, LIDCCEICCNPACFGCLN. This sequence is quite similar to that of the heat-stable enterotoxin (NAG-ST) produced by V. cholerae non-O1 except for one amino acid (leucine) residue excess at the N terminus. Other characteristics, including biological activity, are compatible with those of NAG-ST.

Full text

PDF
2187

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aimoto S., Takao T., Shimonishi Y., Hara S., Takeda T., Takeda Y., Miwatani T. Amino-acid sequence of a heat-stable enterotoxin produced by human enterotoxigenic Escherichia coli. Eur J Biochem. 1982 Dec 15;129(2):257–263. doi: 10.1111/j.1432-1033.1982.tb07047.x. [DOI] [PubMed] [Google Scholar]
  2. Arita M., Honda T., Miwatani T., Takeda T., Takao T., Shimonishi Y. Purification and characterization of a heat-stable enterotoxin of Vibrio mimicus. FEMS Microbiol Lett. 1991 Mar 15;63(1):105–110. doi: 10.1016/0378-1097(91)90536-j. [DOI] [PubMed] [Google Scholar]
  3. Arita M., Takeda T., Honda T., Miwatani T. Purification and characterization of Vibrio cholerae non-O1 heat-stable enterotoxin. Infect Immun. 1986 Apr;52(1):45–49. doi: 10.1128/iai.52.1.45-49.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Edman P., Begg G. A protein sequenator. Eur J Biochem. 1967 Mar;1(1):80–91. doi: 10.1007/978-3-662-25813-2_14. [DOI] [PubMed] [Google Scholar]
  5. Guarino A., Capano G., Malamisura B., Alessio M., Guandalini S., Rubino A. Production of Escherichia coli STa-like heat-stable enterotoxin by Citrobacter freundii isolated from humans. J Clin Microbiol. 1987 Jan;25(1):110–114. doi: 10.1128/jcm.25.1.110-114.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Guarino A., Giannella R., Thompson M. R. Citrobacter freundii produces an 18-amino-acid heat-stable enterotoxin identical to the 18-amino-acid Escherichia coli heat-stable enterotoxin (ST Ia). Infect Immun. 1989 Feb;57(2):649–652. doi: 10.1128/iai.57.2.649-652.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Shimada T., Sakazaki R. A serogroup of non-O1 Vibrio cholerae possessing the Inaba antigen of Vibrio cholerae O1. J Appl Bacteriol. 1988 Feb;64(2):141–144. doi: 10.1111/j.1365-2672.1988.tb02733.x. [DOI] [PubMed] [Google Scholar]
  8. Takao T., Hitouji T., Aimoto S., Shimonishi Y., Hara S., Takeda T., Takeda Y., Miwatani T. Amino acid sequence of a heat-stable enterotoxin isolated from enterotoxigenic Escherichia coli strain 18D. FEBS Lett. 1983 Feb 7;152(1):1–5. doi: 10.1016/0014-5793(83)80469-4. [DOI] [PubMed] [Google Scholar]
  9. Takao T., Shimonishi Y., Kobayashi M., Nishimura O., Arita M., Takeda T., Honda T., Miwatani T. Amino acid sequence of heat-stable enterotoxin produced by Vibrio cholerae non-01. FEBS Lett. 1985 Dec 2;193(2):250–254. doi: 10.1016/0014-5793(85)80163-0. [DOI] [PubMed] [Google Scholar]
  10. Takao T., Tominaga N., Shimonishi Y., Hara S., Inoue T., Miyama A. Primary structure of heat-stable enterotoxin produced by Yersinia enterocolitica. Biochem Biophys Res Commun. 1984 Dec 28;125(3):845–851. doi: 10.1016/0006-291x(84)91360-3. [DOI] [PubMed] [Google Scholar]
  11. Takeda Y., Takeda T., Yano T., Yamamoto K., Miwatani T. Purification and partial characterization of heat-stable enterotoxin of enterotoxigenic Escherichia coli. Infect Immun. 1979 Sep;25(3):978–985. doi: 10.1128/iai.25.3.978-985.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Yoshimura S., Ikemura H., Watanabe H., Aimoto S., Shimonishi Y., Hara S., Takeda T., Miwatani T., Takeda Y. Essential structure for full enterotoxigenic activity of heat-stable enterotoxin produced by enterotoxigenic Escherichia coli. FEBS Lett. 1985 Feb 11;181(1):138–142. doi: 10.1016/0014-5793(85)81129-7. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES