Figure 1.

In situ hybridization for NP1, NP2, and NPR mRNAs in the retinal ganglion cell and inner nuclear layers of the retina. Transverse sections of rat eyes were hybridized with digoxigenin-labeled antisense cRNA probes to NP1 (A), NP2 (B), or NPR (C). D, Hematoxylin and eosin-stained section to reveal cell bodies and retinal layers. E, Sections were also incubated with NP1, NP2, and NPR sense probes. A representative section, with lack of specific signal, is shown here for the NP1 sense probe. F, Low-power image of NP1 mRNA localization, showing lack of a gradient for NP1 expression.