Table 1.
Plasmids and strains used in this study
| Plasmid | Features | Reference |
| prDNAAraA | pBluescript, NTS2::pHXT7tr-AraA(B. subtilis)-tCYC1 | [27] |
| prDNAAraD | pBluescript, NTS2::pHXT7tr-AraD (E. coli)-tCYC1 | [27] |
| pY7 | XYL1 (P. stipitis), XYL2 (P. stipitis), URA3 | [28] |
| YEplacHXT-XIp | pHXT7tr-XI (Pyromyces sp.)-tCYC1, URA3 | [29] |
| YIpAraB | KanMX, pHXT7tr-AraB (E. coli)-tCYC1, TRP1 | [27] |
| YIplac128 | LEU2 | [53] |
| Strain | Genotype | Reference |
| TMB3042 | CEN.PK 2-1C, Δgre3, his3:: p PGK1-XKS1- t PGK1, TAL1:: p PGK1-TAL1- t PGK1, TKL1:: p PGK1-TKL1- t PGK1, RKI1:: p PGK1-RKI1- t PGK1, RPE1:: p PGK1-RPE1- t PGK1, leu2, trp1, ura3 | [31] |
| TMB3070 | TMB3042, YIpAraB | This work |
| TMB3073 | TMB3070, pHXT7tr-AraA(B. subtilis)-tCYC1, NTS2::pHXT7tr-AraD (E. coli)-tCYC1 | This work |
| TMB3074 | TMB3073, YIplac128 | This work |
| TMB3075 (xylose reductase/xylitol dehydrogenase strain) | TMB3074, pY7 | This work |
| TMB3076 (xylose isomerase strain) | TMB3074, YEplacHXT-XIp | This work |